The Collagen (Porcine) Type II, Mouse Antibody (IgG) BioAssay™ ELISA Kit is designed to assay type II collagen antibodies in mouse sera. The microtiter strips provided in the kit have been coated with porcine type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.
This ELISA kit contains enough material to run two plates on two separate occasions (see assay procedure). Figure 1 shows a standard ELISA kit consisting of ten 8-well strips co1vialated with one species of type II collagen and two 8-well strips for reference standards. “B” represents blank wells to determine background values caused by the secondary antibody. Standards and samples (1-39) are run in duplicate.
Kit Components:C7510-33D1: Standard, 1x1 vial (Lyophilized)C7510-33D2: Secondary Antibody (Peroxidase-conjugated goat anti-mouse IgG), 2x1vial (Lyophilized)C7510-33D3: Solution A (Blocking Buffer), 1x12mlC7510-33D4: Solution B (Sample/Standard Dilution Buffer), 1x50mlC7510-33D5: Solution C (Secondary Antibody Dilution Buffer), 20mlC7510-33D6: OPD, 2x1vial (Lyophilized)C7510-33D7: Chromagen Dilution Buffer, 1x20mlC7510-33D8: Stop Solution (2N Sulfuric Acid), 1x10mlC7510-33D9: Type II Collagen-Coated 8-well strips, 10 x 8-well stripsC7510-33D10: Reference Standard Strips (two strips per run), 2 x 8-well stripsC7510-33D11: Wash Buffer, 20X, 1x50ml
Storage and Stability:Store all kit components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
