This ELISA kit is designed to assay type II collagen antibodies in rat sera. The microtiter strips provided in the kit have been coated with bovine type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure).
Kit Components:C7510-33F1: Microtiter Strips, 10x8 wellsC7510-33F2: Reference Microtiter Strips, 4x8 wellsC7510-33F3. Standard Antibody, 1x 20u C7510-33F4: Pab (HRP), 2x1 vial, Dissolve one vial in 10ml of C7510-33F7 Secondary Antibody BufferC7510-33F5: Blocking Buffer, 1x12mlC7510-33F6: Sample/Standard Buffer, 1x60ml, Use for diluting standards and sample specimens
C7510-33F7: HRP Buffer, 1x20mlC7510-33F8: OPD, 2x1 vial, Resuspend 1 vial with 10ml of C7510-33F9: OPD BufferC7510-33F9: OPD Buffer, 1x20ml C7510-33F10: Wash Buffer (20X), 1x50ml, Dilute in 950ml ddH2O
Storage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
