85-1359-59　Phosphatase, Alkaline, Calf Intestine (1,500-2,500u/mg) 1mg　CAS No:9001-78-9　P4071-03B

特徴

• Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. This is a cost effective conjugation grade used in antibody preparation supplied as a lyophilized powder.
• Activity:1,500-2,500 units/mg protein
• Unit Definition:The amount of enzyme which liberates 1umole of p-nitrophenol from p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in diethanolamine buffer.
• Quality Control:SDS-PAGENo contaminating RNase, DNase or Proteases detected
• Activities:PDE ≤0.0004% Adenosine Deaminase ≤0.004% A-5-MP Deaminase ≤0.004%
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Country of Origin:Prepared from US origin starting material and collected in USDA approved facilities.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with distilled water or sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.