85-1359-31　Deoxyribonuclease I (DNase I), 2u/ul, Solution 100U　CAS No:9003-98-9　D3200-02

特徴

• Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3’-hydroxyl oligonucleotides.
• Applications:Nick TranslationDNase I DNA FootprintingCloning of Random DNA Fragments
• Activity:~2000u/ml
• Unit Definition:1 unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0.
• Assay Method:Based on the Kunitz method where DNase will depolymerize DNA; 1 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction. Correlation of digestion units with Kunitz is different for other DNA and buffer systems
• Quality Control:SDS-PAGE
• RNase:None Detected
• Protease:None Detected
• Storage and Stability:May be stored at 4°C for short-term only. Protect from moisture. For long-term storage, store at -20°C. Aliquots with 50% glycerol are stable for 6 months at -20°C.
• Note: Pancreatic DNase is highly susceptible to denaturation. Mix by gentle inversion.