Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000D molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Highly purified type II collagen may be used for assaying antibodies in sera from collagen-induced arthritis (CIA) animal models and humans by ELISA. Polymeric or fibrilar collagen can bind, nonspecifically, to immunoglobulins in serum samples, secondary antibodies and avidin-peroxidase creating high, false-positive reactions. To prevent this, and to obtain consistent assay results, USBio provides highly purified, polymeric-free, ELISA Grade Collagen.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommened Dilutions:ELISA: 5-10ug/ml on ice with chilled Collagen Dilution Buffer (C7510-26A). The optimum concentration of collagen to coat plates varies upon the type of plate used
Note: Recommended to dilute C7510-26 on ice with chilled Collagen Dilution Buffer (C7510-26A) just prior to use
The optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only (1 week). For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Notes:1. C7510-26 will gradually form polymeric collagen and degrade under neutral conditions
2. Do not store diluted solutions of C7510-26.
仕様
Size：500ug
Source Antigen：Bovine sternal cartilage
Grade：Highly Purified
Purity：Highly purified (≥ 99% by SDS-PAGE). Free of pepsin and proteoglycans.
