Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Highly purified type II collagen. Used for the induction of collagen-induced arthritis (CIA) in experimental animals. Suitable for use as an antigen in immunological protocols.
Solubility:Soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve this product in neutral buffer, first dissolve in 0.01 or 0.05M acetic acid, and then add into neutral buffer containing 0.2M NaCl or dialyze against the same buffer.
Note: To avoid fibril formation under neutral conditions, it is recommended to use a buffer containing 0.15-0.2M NaCl and keep on ice. To prepare a collagen gel, dissolve in neutral buffer containing 0.15-0.2M NaCl and incubate it at 37°C for 1-2 hr.
Storage and Stability:Lyophilized powder is stable at 4°C for 2 years and at -20°C for 5 years. Collagen dissolved in acidic buffer is stable at 4°C for 1 month and at -20°C for 1 year, but will gradually degrade under neutral conditions. Protect from light.
Related products:Type I Collagen, ELISA GradeType II Collagen ELISA GradeType II Collagen AdjuvantType II Collagen ELISA Kits
仕様
Size：5mg
Source Antigen：Rat sternal cartilage
Grade：Highly Purified
Purity：≥ 99% by SDS-PAGE
Form：Supplied as a lyophilized powder. Soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve this product in neutral buffer, first dissolve in 0.01 or 0.05M acetic acid, and then add into neutral buffer containing 0.2M NaCl or dialyze against the same buffer. To avoid fibril formation under neutral conditions, it is recommended to use a buffer containing 0.15-0.2M NaCl and keep on ice. To prepare a collagen gel, dissolve in neutral buffer containing 0.15-0.2M NaCl and incubate it at 37°C for 1-2 hr.
