85-1358-89　Histone H1 (F1), Lysine Rich 10mg　H5110-07

特徴

• Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers
• With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature.
• Molecular Weights of Histones:Lysine Rich (H1, f1): ~ 31,500 Slightly Lysine Rich (H2a, f2a2): 14,004 Slightly Lysine Rich (H2b, f2b): 13,774 Arginine Rich (H3, f3): 15,324 Arginine Rich (H4, f2a1): 11,282
• Description:Histone subclass F1
• Specificity:SDS-PAGE Analysis: Doublet at ~31kD (GE Healthcare PhastGel Homogenous 20%)
• Instructions for Use:Most soluble in an acidic buffer such as 0.9M acetic acid at 1mg/ml. (Some assay systems may require more or less dilute histones.) Allow sufficient time for complete dissolution. Do not store diluted histones, as they will lose activity in solution. Prepare histone solution immediately before use.
• Storage and Stability:Store lyophilized histones desiccated at 4°C. Reconstitute per reconstitution instructions. Do not store reconstituted histones. Histones lose activity in solutions. Further dilutions can be made in assay buffer.