Formulation (g/L):Enzymatic Digest of Casein 10.0Enzymatic Digest of Gelatin 10.0Yeast Extract 5.0Sodium Chloride 5.0Dextrose 1.0L-Arginine 1.0Sodium Pyruvate 1.0Hemin 0.0050Vitamin K 0.0005Total 33.0
Directions per Liter:Dissolve 33g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave ((15psi) for 15 minutes at 121ºC.
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.