Selective medium for Coliform organisms, based on lactose fermentation, from urine and routine water and food testing. Lactose fermenting bacteria appear as yellow colonies. Lactose non-fermenting bacteria appear as blue colonies.
Appearance:Light green, homogeneous, free flowing powder with soft lumps.
pH (3.3%, 25ºC):6.9±0.2
Formulation (g/L):Peptic Digest of Animal Tissue 2.5Pancreatic Digest of Casein 2.5Yeast Extract 3.0Lactose 10.0Tergitol 7 0.1Bromthymol Blue 0.025Agar 15.0 Total: 33g/L
Directions per Liter:Dissolve 33g in 1L of ddH2O stirring gently with heating until completely solubilized. Boil for one minute to dissolve completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). After cooling medium to 45-50.C add 3.0 ml of one percent triphenyl tetrazolium chloride, if desired.
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
