Highly nutritive medium for use in isolation of fastidious anaerobic organisms when supplemented.
Appearance:Beige, homogenous, free-flowing powder
Solubility:Light beige to amber, trace to light hazy,
pH:7.6 ± 0.2
Performance Specification:Bacterpides fragilis ATCC 19615: Good to excellentBacteroides vulgatus ATCC 8482: Good to excellentClostridium perfringens ATCC 13124: Good to excellentStreptococcis pyogenes ATCC 19615: Good to excellent
Directions per Liter:Dissolve 41.9g in 800-900ml of ddH2O stirring with heating frequent agitation and boil for one minute to completely. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and StabilityStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
