Enhances Pseudomonas fluorescein production. Recommended in USP for microbial limit tests.
Microbiological Analysis:Note: Growth results obtained from type cultures after incubation at 37°C and observed after 18-24 hours.
Pseudomonas aeruginosa ATCC 9027; Good GrowthPseudomonas aeruginosa ATCC 10145; Good GrowthPseudomonas aeruginosa ATCC 27853; Good GrowthEscherichia coli ATCC 25922; Good Growth
Components shown as g/L:Tryptone 20.0gAgar 15.0gPotassium Sulfate 10.0gMagnesium Chloride 1.4gTotal: 46.4g/literDirections per Liter:Dissolve 46.4g and 10g of glycerol in 800-900ml of dH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.