Mueller Hinton Agar is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to National Committee for Clinical Laboratory Standards (NCCLS).
Quality Control:Tested according to USB Standard Test Methods and NCCLS M6-A. Testing was performed in compliance with ISO/TS 11133-1 and BS EN 12322:1999 + A1:2001 standards. This product met or exceeded established NCCLS performance specifications.
Appearance:Beige, homogenous, free-flowing powder
Solubility:Light to medium yellow, clear, complete after autoclaving.
pH:7.3 ± 0.1
Directions per Liter:Dissolve 38 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
