Enumeration of bacteria by the membrane filter method.
Appearance:Supplied as a light beige, homogenous, free flowing powder
Solubility:Yellow to gold, clear, complete
pH:7.0±0.2
Cultural Response:The medium was prepared and inoculated with the organisms listed below. Cultures were incubated aerobically at 35±2ºC and examined for growth at 18-24 hours.
Formulation (g/L):Enzymatic Digest of Casein 10gBeef Extract 6gDextrose 2gTotal18g/L
Directions per Liter:Dissolve 18 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
