MacConkey agar is a selective and differential medium for detection of coliforms and other enteric organisms.
Appearance:Beige with pale pink tint, homogenous, free-flowing powder.
pH (5%, 25ºC):7.1 ± 0.2
Microbiology:The medium was prepared according to formulation and inoculated with the organisms listed below. Cultures were incubated at 35±2°C in an aerobic atmosphere and examined for growth at 18-24 hours.
Enterococcus faecalis ATCC 29212:Partial to complete inhibition
Escherichia coli ATCC 25922:Growth
Proteus mirabilis ATCC 12453:Growth, partial inhibition of swarming
Salmonella typhimuriumATCC 14028:Growth
Directions per Liter:Dissolve 50 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
