Standard methods medium for growth of coliform bacteria in water and food.
Lauryl Sulfate Broth was formulated by Malimann and Darby (1) for detection of coliform bacteria in water. This is a selective medium because it contains sodium lauryl sulfate which inhibits Gram positive microorganisms. Lactose is added to detect rapid lactose fermentation by coliforms.
Quality Control Summary:Expected cultural response after 18-24 hours at 35.0°C..
OrganismResultEnterobacter aerogenes ATCC 1304Growth, Gas +8 Escherichia coli ATCC 25922 Growth, Gas +Proteus mirabilis ATCC 12453 Growth, Gas -Salmonella typhimurium ATCC 14028Growth, Gas -Staphylococcus aureus ATCC 25923No Growth
Appearance: White to off-white crystalline powderSolubility: Clear, light amber and completepH: 6.8± 0.2
