Assists in differentiation of microorganisms, especially Staphylococcus aureus and Serratia marcesens.
Description:DNase Test Agar with Toluidine Blue is a complete medium since toluidine blue is incorporated in the formulation. A positive reaction is indicated by a rose-pink zone surrounding the colony in a dark blue background. In general, Gram positive microorganisms are inhibited. This medium is particularly useful for determining DNase activity of the Enterobacteriaceae.
Appearance:Light tan with blue specs, free flowing powder
Solubility:Blue, clear, complete after autoclaving
pH:As Reported
Directions per Liter:Dissolve 42.1 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability:Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
