Brain-Heart Infusion Solids is dehydrated infusion of porcine brains and hearts for use in preparing microbiological culture media. Rosenow devised an excellent medium for culturing streptococci by supplementing Dextrose Broth with bovine brain tissue.1 Hayden, revising Rosenow’s procedure by adding crushed marble to the medium, reported favorable growth of organisms from dental pathogens. Brain-Heart Infusion Solids was developed as an alternative to bovine based Brain Heart Infusion containing media, replacing calf brain and beef heart with porcine brains and hearts. Brain-Heart Infusion Solids was developed for pharmaceutical and vaccine production, and can replace traditional bovine based BHI media in most applications. The nutritionally rich formula of Brain-Heart Infusion Solids is used to grow a variety of microorganisms. The original Brain-Heart Infusion media are specified in standard methods for multiple applications.3-6
Brain-Heart Infusion Solids provides nitrogen, amino acids, and vitamins in microbiological culture media. Brain-Heart Infusion Solids is processed from large volumes of raw material, retaining all the nutritive and growth stimulating properties of fresh tissue.
Precaution:1. For Laboratory Use
Appearance:Yellow to beige, free flowing, homogenous powder
Solubility (2%):Brilliant to clear, amber solution which may or may not have a light precipitate
pH (2%):6.5-7.5
Loss on Drying:≤6.0%
Total Nitrogen:11-13%
Ash:<15%
Standard Plate Count:≤5000cfu/g
Expected Cultural Response:Cultural response on 2% Peptone Agar at 35°C after 18-24 hours incubation.
Peptone Agar (2%):E. coli ATCC 25922: good to excellent growthS. aureus ATCC 6538: fair to good growth
Country of Origin:Brain: Porcine, USA/MexicoHeart: Porcine, USA/Mexico
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
