Description:In 1926 Wilson and Blair used Bismuth and Sodium Sulfite in a culture formulation for the isolation of typhoid-paratyphoid groups of bacteria bacteria. Further modifications of the formula were described by Hajna and Damon. The current recommended formulation is found in the USP. Bismuth and brilliant green dye acts as inhibitors to most enteric bacteria and Gram positive bacteria. Salmonella typhi produces black colonies surrounded by a metallic sheen. Other Salmonella produce green or black colonies. Some Shigella sp. may grow on this medium.
Storage:Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
Microbiological Analysis:Escherichia coli (ATCC 25922): Partial Inhibition Brown to GreenEnterococcus faecalis (ATCC 29212): InhibitedSalmonella typhi (ATCC 19430): GoodBlack with metallic sheenSalmonella typhimurium (ATCC 14028): Good Black-Greenish/GreyShigella flexneri (ATCC 12022): Partial Inhibition Greenish-Brown
Appearance: Light beige, homogeneous and free flowing
Solubility: Greenish-yellow and opaque.
pH: 7.5± 0.2
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
