Differentiation of Group D from non-Group D Streptococci.
Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Gram positive bacteria other than enterococci are inhibited by the bile salts.
Appearance: Light beige, homogeneous and free flowing
Solubility: Dark amber and turbid to clear.
pH: 6.6 ± 0.2
Microbiological Analysis:Enterococcus faecalis (ATCC19433); Good Black colonies and mediumEnterococcus faecalis (ATCC 29212); Good Black colonies and mediumEnterococcus faecalis (ATCC33186); Good Black colonies and mediumEscherichia coli (ATCC 25922); Good Colorless No blackeningStreptococcus pyogenes (ATCC 19615); Inhibited N/A
*The results were obtained from type cultures after incubation at a temperature of 35ºC and observed after 18-24 hours.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
