85-1355-46　Triticum vulgare (WGA, Wheat germ) (Biotin) 1mg　T8653-15

特徴

• The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Biotinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
• Source:Wheat germ
• Blood Specificity:A, B, O
• Carbohydrate Specificity:N-Acetylglucosamine
• Inhibitory Carbohydrate:Chitin Hydrolysate or N-Acetylglucosamine
• Divalent Ions:None
• Applications:Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
• Recommended Dilutions:For most applications we recommend a freshly prepared working solution of 5-20ug/ml in the above buffer.
• Activity:<4mg/ml will agglutinate human type O erythrocytes. <1ug/ml will agglutinate neuraminidase treated erythrocytes.
• Quality Control:SDS-PAGE
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
• Toxicity and Hazards:All products should be handled by qualified personnel only, trained in laboratory procedures.