Hemoglobin is composed of one globin plus four hemes. Heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520. Ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
No reaction against any glycohemoglobins, including HbA1a, HbA1b, HbF and HbA1c.
Substitution:≤0.05 glyco groups/monomer Hb
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
