Hemoglobin is composed of one globin plus four hemes. Heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520. Ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
Contaminants:No reaction against any glycohemoglobins, including HbA1a, HbA1b, HbF and HbAo.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Source Antigen:Human red blood cells
Grade:Purified
Purity:~80% (HPLC)
Form:Supplied as a frozen liquid in 1mM KCN buffer, pH 7.5. One glyco group per β-chain monomer N-terminal valine.