85-1353-10　［取扱停止］Creatine Kinase BB Isoenzyme (CKBB) 100U　C7910-14

特徴

• Creatine kinase (CK) is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). This CK enzyme reaction is reversible, such that also ATP can be generated from PCr and ADP.
• Appearance:Supplied as a liquid in Imidazole, DTT, pH 6.5, 50% glycerol.
• Purity:≥99%
• Activity:≥500U/ml
• Specific Activity (U/mg protein):As reported
• Concentration:~0.5mg/ml Protein
• Unit Definition:One unit transfers 1 micromole of phosphate from creatine phosphate to ADP/minute at 37ºC.
• CK-BB:≥99%
• CK-MM:≤1%
• CK-MB:≤1%
• LDH:≤0.01%
• AST/GOT:≤0.01%
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.