Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Specific Activity:≥10u/mg enzyme activity
Unit Definition:One unit is defined as the amount of enzyme that will hydrolyze 1.0umol Z-RR-pNA per min at 37°C, pH 6.8 in the presence of DTT. Note: One unit defined by Z-RR-pNA equals ~20 units defined with Z-RR-betaNA.
Major Bands:25-27kD, 30-33kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Human liver
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 50mM sodium acetate, pH 5.0, 1mM EDTA.
