Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
Unit Definition:1 unit causes an increase in absorbance at 260nm of 0.001 per minute per minute at 25ºC, pH 4.6 using highly polymerized DNA as substrate.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20KU
Source Antigen：Porcine spleen
Grade：Molecular Biology Grade
Purity：Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
Form：Supplied as a chromatographically purified, dialyzed, lyophilized powder.
