85-1351-21　ImmunoTek™ ABTS Stable Liquid Substrate 1.46mM for HRPO 1000ml　A0030

特徴

• ImmunoTek™ ABTS Stable Liquid Substrate 1.46mM for HRPO
• ABTS is a soluble, safe, convenient, and good overall performing substrate for horseradish peroxidase (HRP) based ELISA assays. ABTS develops an intense blue-green color measurable between 405nm and 410nm. It may be used for both kinetic and endpoint reactions
• ABTS (1.46mM) is supplied in citrate buffer, pH 4.0.
• In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation showing absorption maxima at 820nm, 734nm, 650nm, and 405nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays.
• Stopping the reaction with acid does not alter the 405nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength.
• Using a proprietary, nontoxic stabilization technology, United States Biological provides a room temperature stable, single component ABTS solution satisfactory for quantitative analysis of HRP based systems.
• Features:
• Single component system
• Room temperature stable
• Exhibits low background levels
• Safe to use-no weighing of hazardous powders
• Save preparation time-use right out of the bottle
• Ultra-low sensitivity
• Minimal lot-to-lot variation
• Appearance:Colorless to very pale green liquid
• pH:4.0 ± 0.05
• Storage and Stability:May be stored at RT or at 4ºC. Stable for 12 months after receipt at 4ºC.