Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors.
Used in protein sequencing and tissue disassociation.
Unit Definition:1 unit hydrolyzes 1umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25ºC, pH 8.2, in the presence of 0.01M calcium ion.
Quality Control:SDS-PAGE
Certificate of Origin:Trypsin is derived from animal products of North American origin, collected in USDA-approved facilities and inspected to be free of disease.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or media. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100mg
Source Antigen：Bovine Pancreas
Grade：Molecular Biology Grade
Purity：Chromatographically purified
Form：Supplied as a lyophilized powder. Trypsin has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity. Trypsin is also irradiated and tested for the absence of mycoplasma and extraneous virus. Reconstitute with 10ml of sterile buffer or media (Hanks, Earles, BSS, PBS, etc.).
