Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors.
Unit Definition:1 unit of trypsin hydrolyzes 1umole of p-toluenesulfonyl-L-arginine methyl ester (TAME) per minute @ 25ºC, pH 8.2, in the presence of 0.01M calcium ion.
Appliations:Sutiable for use in Protein Sequencing, Cell and Tissue Disassociation. Other applications not tested.
Recommended Dilutions:Monolayer Dissociation: 0.002-0.01% depending upon strain
Primary Embryo Tissues: 0.01-0.1%Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute to nominal volume,aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin: North America
Certificate of OriginThe raw animal product used in the manufacturing of Trypsin is derived from materials originating in North America. Material is processed in a USDA inspected facility.
Note:Trypsin is subject to a pH ≤3.0 for ≥5 hours during processing.
仕様
Size：100mg
Source Antigen：Bovine Pancreas
Grade：Molecular Biology Grade
Form：Supplied as a sterile-irradiated, 2X lyophilized, crystalline powder.
