Collagenase is prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suitable for use in pancreatic islet primary isolation and other applications where receptor integrity is crucial.
Cofactors:Zinc, Calcium
Collagenase nomenclature:Type 1: Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells
Type 2: Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells
Type 3: Containing low proteolytic activity. It is usually used for mammary and fetal cells
Type 4: Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.
Source:Clostridium histolyticum
Form:Supplied as a dialyzed, lyophilized powder.
Sterility:Filtered through a 0.22um filter.
Quality Control:SDS-PAGE
Activity:≥160u/mg dry weight.
Unit Definition:1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
Storage and Stability:Lyophilized powder may be stored desiccated at 4°C or -20°C. Lyophilized powder stability at 4°C is 12 months. Stability at -20°C is > 12 months.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
