Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
Collagenase is prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suitable for use in pancreatic islet primary isolation and other applications where receptor integrity is crucial.
Cofactors:Zinc, Calcium
Collagenase nomenclature:Type 1 Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells
Type 2 Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells
Type 3 Containing low proteolytic activity. It is usually used for mammary and fetal cells
Type 4 Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.
Source:Clostridium histolyticum
Form:Supplied as a dialyzed, lyophilized powder.
Activity:≥100units/mg dry weight.
Unit Definition:1 unit releases 1 µmole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Quality Control:SDS-PAGE
Storage and Stability:Collagenase is stable for 1-2 years at 2-8ºC. Storage at -20ºC (desiccated) is also acceptable.
