Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
Collagenase is prepared to contain higher clostripain activity levels. Suitable for use in heart, bone, thyroid, salivary and liver primary cell isolation.
Collagenase nomenclature:Type 1: Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells
Type 2: Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells
Type 3: Containing low proteolytic activity. It is usually used for mammary and fetal cells
Type 4: Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.
Source:Clostridium histolyticum
Activity (dw):≥125u/mg dry weight
Units/Vial:≥6250u/vial
Units/mg Caseinase (dw):≥200u/mg dry weight
Units/mg Clostripain (dw):≥3.5u/mg dry weight
Units/mg Tryptic (dw):≥3.5u/mg dry weight
Unit Definition:1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5
Absorbance @ 280nm:As Reported
Colony Count:≤1cfu/ml at 37°C, 2ml recon., 48 hrs.
Quality Control:SDS-PAGE
Sterility:Filtered through a 0.22 micron membrane
Form:Supplied as a dialyzed, lyophilized powder
Storage and Stability:Store lyophilized powder desiccated at 4°C. Storage at -20ºC (desiccated) is acceptable.
