Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
Activity:≥125 units/mg dry weight
Caseinase: As reportedClostripain: As reportedTryptic: As reported
Unit Definition:1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Absorbance (A280):As Reported
Collagenase nomenclature:Type 1: Crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities
Type 2: Contains higher relative levels of protease activity, particularly clostripainType 3: Contains lowest levels of secondary proteasesType 4: Designed to be especially low in tryptic activity to limit damage to membrane proteins
Storage and Stability:Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
