85-1349-93　Collagenase, Type I, C. histolyticum 100mg　CAS No:9001-12-1　C7511-10

特徴

• Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
• Collagenase nomenclature:Type 1. Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells
• Type 2. Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells
• Type 3. Containing low proteolytic activity. It is usually used for mammary and fetal cells
• Type 4. Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.
• Specific Activities:Collagenase:≥125 units/mg dry weight
• Caseinase:≥200u/mg dry weight
• Clostripain:≤4.0u/mg dry weight
• Tryptic:≤0.5u/mg dry weight
• Absorbance:As reported
• Unit Definition:1 unit releases 1 micromole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
• Quality Control:SDS-PAGE
• Storage and Stability: Collagenase is stable for 6 months after receipt at 4°C. Storage at -20°C (desiccated) is also acceptable.