85-1349-52　TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder) 100L　T2000

特徴

• TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda.
• TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
• Appearance:White to off white, homogenous, free flowing powder. Includes NaHCO3
• Solubility:Colorless to light yellow, clear, complete.
• pH:6.5 ± 0.5
• Directions per Liter:Dissolve 19.4 grams in 800-900 mL of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
• Note: On occasion, this formulation seems reluctant to dissolve. If continuous stirring with warmth has not produced complete dissolution, add 1.0N HCl slowly until solution turns crystal clear with light yellowish tone (pH 4.2-4.5). Let solution cool to RT, then back titrate with 1.0N NaOH to pH 6.2-6.4. Components will remain solubilized.
• Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.