85-1344-72 [取扱停止]Leibovitz L-15 Medium w/L-Glutamine, HEPES, Tris, Low Sodium (Powder) 50L L2100
特徴
- The most commonly used buffering system for mammalian cell cultures is a bicarbonate/CO2 system, which requires CO2 regulators and incubators to supply a constant level of CO2. As a replacement, Leibovitz developed a bicarbonate-free medium, L15, with relatively high levels of certain amino acids in the free base form.
- The most common method in use today for buffering mammalian cell cultures is the bicarbonate/CO2 system, which is based on the following equilibrium:CO2 + H2O <-> HCO3" + H+
- The use of bicarbonate is an attempt to mimic the buffering system of blood, but it has at least two major drawbacks. (1) The p/Q of bicarbonate is 6*1, which is far removed from the desired pH range of cell culture media (7-0-7-4). (2) Although bicarbonate is cheap, supplying a constant level of CO2 to cell cultures is definitely not, as it requires expensive CO2 incubators or fermentors.
仕様
- Size:50L
- Grade:Cell Culture Grade
- EU Commodity Code:38210000
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