Purified from an E.coli strain carrying a T4 RNA ligase overproducing plasmid. Catalyses the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of single-stranded RNA or DNA. The minimal substrate is a nucleoside 3', 5'-diphosphate.
ApplicationsRNA 3'-end labelling (1)Joining RNA to RNA (2)Circularization of oligonucleotides (3)Joining synthetic oligodeoxyribonucleotides (4)Specific modifications of tRNAs (5)Oligodeoxyribonucleotide ligation to single-stranded cDNAs (6)Generation of composite primers to PCR (7)
