Ampicillin Beta-Lactamase Modified, is a unique antibiotic that is a stronger inhibitor of b-Lactamase than ampicillin, carbenicillin or methicillin. Ampicillin is modified utilizing a novel method that results in improved transformation efficiencies where higher cell density and longer incubation times traditionally result in the development of ampicillin sensitive satellite colonies. When used at a concentration of 1X, the presence of satellite colonies Identificatis reduced, even after prolonged incubation (2-4 days) and plating of competent cells at 20 fold higher density.
Advantages:
No background (satellite) colonies are observed
Allows higher density plating
Longer incubation times are readily achieved at 37°C
Supplied as a convenient 1000X solution
Quality Control:E. coli DH5a cells were transformed with pUC19 DNA and plated on LB agar plates containing 150µg ampicillin and 1X Ampicillin Beta-Lactamase Modified and incubated at 37°C for 24 hours. The plate containing ampicillin had greater than 103 satellite colonies while the plate containing Ampicillin, Beta-Lactamase Modified™ showed no satellite colonies.
Components:Ampicillin Beta-Lactamase Modified 1000X , 3 x 1ml.
Instruction for Use:b-Lactamase Modified Ampicillin is supplied as a ready solution at a concentration of 1000X
To use, add 1ml per liter of the medium. Do not add to the medium at temperatures above 60°C.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
