85-1342-29　Leupeptin Hemisulfate 5mg　CAS No:103476-89-7　L2050

特徴

• Leupeptin is a reversible competitive inhibitor of serine and thiol proteases that competes with substrate for the active site of the enzyme. It has been reported to inhibit calpain, cathepsin B and trypsin, for example. Leupeptin is often used for the inhibition of plasmin, trypsin, papain, kallikrein and cathepsin B. No inhibition found with pepsin, cathepsins A and D, elastase, renin or chymotrypsin. A typical working concentration is in the range of 10-100uM
• Leupeptin has 3 tautomeric isomers which are separated by chromatography.
• Synonyms:Microbial Leupeptin; Acetyl-Leu-Leu-Arg-al; Leupeptin hemisulfate salt; N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
• Molecular Formula:C20H38N6O4•1/2H2SO4
• Molecular Weight (Sulfate Form) (Ac-Leu-Leu-Argininal):426.55
• Appearance:White powder
• Optical Rotation:-80.0° ± 2°
• Water:≤5%
• Specific Activity (Trypsin/TAME):~1.2ug/ml
• Solubility:Soluble in water, ethanol, acetic acid and DMF. Clear to v.slightly hazy solution at 50mg/mL in water.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Product is stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.