85-1341-67　Nematode Growth Medium (NGM) (Powder) 500g　N1000

特徴

• A standard preparation for use in Nematode growth studies.
• Appearance:Light tan to pale yellow, homogeneous, free flowing powder
• Solubility without autoclaving:Light tan, incomplete
• Solubility with autoclaving:Light tan, clear, complete
• pH with autoclaving:As Reported
• Protocol:Cooling the N1000 solution after autoclaving:Place the sterile N1000 solution into a 58°C water bath. N1000 solution needs to completely cool to the desired temperature (58°C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar.
• Further preparing the medium:Once the N1000 solution has cooled to 58°C, place flask(s) onto stir plate(s). Maintain temperature at 58°C. Add the following:a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solutionb)Sterile 1M CaCl2: 1ml/liter N1000 solutionc) Sterile 1M MgSO4: 1ml/liter N1000 solutionAllow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved
• *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L).