85-1341-45　［受注停止］Nocodazole 250mg　CAS No:31430-18-9　N3000

特徴

• Nocodazole, an inhibitor of microtubule formation, was found to greatly inhibit cell division around the mound stage as well as during the vegetative growth phase, when applied to exponentially growing Ax-2 cells. Mitotic arrest of cells with nocodazole can be used to study phosphorylation of microtubule-associated proteins which increase during mitosis, concomitant with increased microtubule dynamics. Nocodazole has been found to dramatically inhibit T cell antigen receptor (TCR) signaling, independently of its ability to depolymerize microtubules. Nocodazole arrests the cell cycle at G2/M phase and prevents phosphorylation of the T cell antigen receptor and inhibits its activity.
• Synonyms:Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate; Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate; Oncodazole; R 17934; [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamic acid methyl ester
• Molecular Formula:C14H11N3O3S
• Molecular Weight:301.32
• Appearance:Supplied as a light orange to brown powder
• Purity:≥98% (HPLC)
• Melting Point:295-305ºC
• Method for Determining Identity:Proton NMR Spectroscopic Analysis
• Solvent Solubility (g/100ml):DMSO: 1 (with warming)Formic Acid: 15.30Acetic Acid: 1.39Lactic Acid: 1.79PEG 400: 0.80Methanol: 0.026NaOH, 0.1N, pH 12.3: 0.015Ethanol: 0.011Water: < 0.0005HCl, 0.1N, pH 1.2: 0.007Citrate-Phosphate Buffer, pH 8.0: 0.0001Note: Nocodozole is relatively insoluble.
• Note: Nocodozole is relatively insoluble.
• Usage:Prepare stock solution at 5mg/ml in DMSO. Further dilutions can be made in culture media. Due to insolubility (nocodazole is only slightly soluble in water), no more than 10ug/ml concentration can be used in cell culture systems. Nocodazole is stable in biological media for seven days.
• Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
• Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.