85-1340-06　DNA Polymerase I Klenow Fragment 1500U　CAS No:9012-90-2　D3940

特徴

• DNA Polymerase I Large Fragment exhibits the 5’ ---> 3’ polymerase and 3’ ---> 5’ exonuclease activity of DNA Polymerase. Lacks 5’ ---> 3’ exonuclease activity.
• Applications:
• Random primer labeling
• Sanger sequencing
• cDNA Second Strand Synthesis
• 3’ end-labeling, filling
• Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol
• Oligonucleotide-directed site-specific mutagenesis.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：1500U
• Source Antigen：E. coli cells with a cloned fragment of the polA gene
• Grade：Molecular Biology Grade
• Form：Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 0.1mM EDTA, 1mM DTT, 50% glycerol.
• Molecular Weight Non Ab：62
• EU Commodity Code：38220090
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• 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。
• CAS No ：9012-90-2

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