85-1339-33　Bromodeoxyuridine (BrdU) 25g　CAS No:59-14-3　B2850

特徴

• 5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations. Bromodeoxyuridine (BrdU) is also used to prepare dNTP-BrdU solution for UV cross-linking of proteins to nucleic acids and to prepare complete MEM-10-Brdu or MEM-20-Brdu for vaccinia virus stock cultures.
• Synonyms:5-Bromo-2’-deoxyuridine; 5-BrdU; 2’-Deoxy-5-bromorouridine; (+)-5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; Bromodeoxyuridine; Broxuridine; NSC 38297
• Molecular Formula:C9H11BrN2O5
• Molecular Weight:307.10
• Appearance:White to off white, crystalline or powder
• Purity (HPLC):≥98%
• UV Content (on dry basis):≥97%
• Ratio of Absorbances (pH2):0.47-0.53 (A250/A260)1.87-1.93 (A280/A260)1.45-1.55 (A290/A260)
• Melting Point:~187-189°C
• Specific Rotation (C=1%, H2O):+23.0°
• Water (KF):≤1%
• Solubility:Soluble in 0.1N NaOH and DMF, sparingly soluble in water, slightly soluble in ethanol and acetone, practically insoluble in ether
• RNase:None Detected
• DNase:None Detected
• Protease:None Detected
• Endotoxin:≤0.5EU/mg