85-1336-53　［受注停止］Gentamycin (Gentamicin) Sulfate USP 25g　CAS No:1405-41-0　G2030

特徴

• Broad spectrum, cell culture aminoglycoside antibiotic that is non toxic to viruses and mammalian cells at antibacterial levels. Inhibits growth of gram positive and negative bacteria, and mycoplasma. Inhibits normal protein synthesis by binding to L6 protein of 50S ribosomal subunit. Useful antibiotic for Baculovirus expression systems since mutations in rplF prevent Gentamycin from binding.
• Causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site. Antibacterial against Gram-negative aerobic bacteria, Gram-positive bacteria and mycoplasmas. Used as a selection agent (gentamicin-resistance gene) in molecular biology applications. Broad-spectrum cell culture antibiotic that is nontoxic to viruses and mammalian cells at antibacterial and antimycoplasmal concentrations. Due to its extended stability and slow development of bacterial resistance, it is a useful antibiotic in long-term virus und tissue culture studies.
• Bactericidal effects are exerted by the binding to the outer membrane, causing disruption of the membrane. This increases the permeability of the cell envelope, leakage of cell contents, and leading to apoptosis and proteolysis (cell death). Causes also cell death by generation of free radicals, phospholipidosis, extracellular calcium-sensing recep- tor stimulation and energetic catastrophe.
• Source:Micromonospora sp.
• Synonyms:Alcomici; Bristagen; Cidomycin; Duragentam; Garamycin; Garasol; Apogen, Gentiomycin C, Refobacin, NSC-82261; Gentamycin sulphate
• Molecular Formula:C21H43N5O7.H2SO4
• Molecular Weight:~575.6
• Appearance:White to off-white powder
• Potency:≥590ug Gentamycin/mg
• Gentamycin Content:C1: 25-50%C1a: 10-35%C2a+C2: 25-55%
• Identity (IR):Conform to reference
• Specific Rotation:+107° to +121°
• pH:3.5-5.5
• Loss on Drying:≤15%
• Residue on Ignition:≤1%
• Working Concentration:15-30ug/ml
• Endotoxin:≤0.5EU/mg
• Inhibition:Inhibits growth of JM109 and S. aureus
• Storage and Stability:Store at 4°C. Stable for 12 months after receipt.
• Meets or exceeds USP specifications