85-1335-41　Agarose High Gel Temp 50g　CAS No:9012-36-6　A1025

特徴

• High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications.
• Applications:
• Pulsed Field Electrophoresis
• Immunoelectrophoresis
• Blotting Assays (Southern, northern)
• Analytical DNA Gels ≥ 1000bp
• Preparative Electrophoresis
• For use with all buffer systems
• EEO:≤0.14
• Melting Point (1.5%): 85.5-88.5°C
• Gel Point (1.5%): 39.5-42.5°C
• Gel Strength (1.5%): ≥1200g/cm2
• Clarity:≤50 NTU
• Sulfate:≤0.2%
• RNase:None Detected
• DNase:None Detected
• Quality Control:Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
• Functional and Molecular Biology Testing: To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23,000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
• Recommended Usage (1X TAE):ConcentrationMolecular Weight0.4-2.0%50-500kb
• Storage and Stability:Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.