85-1335-21　［取扱停止］Agarose DNA Fragments 150-1500bp 100g　CAS No:9012-36-6　A1010

特徴

• Agarose for DNA Fragments 150-1500bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
• Manufactured specifically for primer size fragments in the range of 150-1500 base pairs and exhibits exceptionally high transparency even at high concentrations (4.5%).
• Applications:
• Analytical DNA Gels ≤ 1500bp
• PCR products
• Restriction Enzyme digestion products
• Mutation Analysis
• Blotting Assays ≤ 600bp
• For use with all buffer systems
• Recommended Usage (1X TAE):ConcentrationMolecular Weight4%150-600 bp 12%500-1500 bp
• Source:Gelidium
• Form:White, homogeeous free flowing powder
• Clarity (NTU, 1.5%):~1.5
• Colorimetry (A450):≤ 0.02
• pH in solution (1.5%):5.5-7.0
• pH in gel (1.5%):5.5-7.0
• Gel Strength (1.5%):≥2000g/cm2
• Gel Strength (4%):≥4250g/cm2
• Gel Point (4%):≤40°C
• Melting Point (4%):≤89ºC
• EEO:≤0.12
• Loss on Drying:≤7.0%
• Ash:≤0.35%
• Sulfate:≤0.1%
• RNase:None Detected
• DNase:None Detected
• Quality Control:Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
• Functional and Molecular Biology Testing:To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23,000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
• Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.