85-0160-33　［取扱停止］Rat Rectal Smooth Muscle Cells 5x10^5 cells/vial　EP-CP-R042

特徴

• 背景：Rat rectal smooth muscle cells were isolated from rectal tissue. The rectum is the last part of the large intestine, located in the small pelvis. The sigmoid colon is connected to the third sacral vertebra at the upper end, descending along the front of the sacrum and coccyx, passing through the pelvic diaphragm, and ending in the anus at the lower end. The upper end of the rectum is similar in size to the colon, and its lower end expands into the ampulla of the rectum, which is the temporary storage site for stool before it is discharged, and the lower end becomes thinner to connect to the anal canal. The position of the rectum in the pelvic cavity is closely related to the ventral surface of the sacral spine, and has the same curvature as the sacral spine. There is fat around the rectum, no longitudinal bands, and it is located on the back of the bladder and reproductive organs. After 3 days of primary isolation and culture of rectal smooth muscle cells, the cells can be seen to adhere to the flask, and the cell morphology and size are different. Regular, triangular or fan-shaped, with oval and centered nucleus. The cells reach confluence after 2 weeks, most of the cells stretch out in a long fusiform shape, with abundant cytoplasm and branch-like protrusions. The cells are arranged in a single layer or partially overlapped in multiple layers growth, ups and downs. When the cell density is low, they are often intertwined into a network; when the density is high, they are arranged in a whirlpool or fence shape. After passage, the cells grow faster and reach confluence in 4-6 days and maintain the above-mentioned morphological characteristics and growth characteristics.The arterial blood supply of the rectum mainly comes from the superior rectal artery of the inferior mesenteric artery, the middle rectal artery of the internal iliac artery, and the inferior rectal artery of the internal iliac artery. Smooth muscle contraction is the basic mode of movement in gastrointestinal peristalsis. In enteritis, the smooth muscle layer thickens due to the increase of smooth muscle special actin. Smooth muscle actin may affect the production of contractile force, which further proves that intestinal smooth muscle cells have plasticity during inflammation. The culture of intestinal smooth muscle cells can help understand the contraction, proliferation, and response of gastrointestinal connective tissue to smooth muscle cells. The rat rectal smooth muscle cells produced by our company are prepared by the trypsin-collagenase combined digestion method and the differential adhesion method. The total number of cells is about 5×10^5 cells/vial. The cells are identified by alpha-SMA Immunofluorescence identification, the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, etc.