85-0160-01　［取扱停止］Rat Lung Fibroblast 5x10^5 cells/vial　EP-CP-R006

特徴

• 背景：Rat lung fibroblasts are isolated from lung tissue. The lung is the respiratory organ of the body, located in the chest cavity, one on the left and one on the left, covering the heart. The lung has lobes, two on the left, three on the right, and a total of five lobes. The lung passes through the lung system(Referring to the trachea, bronchi, etc.), connected to the larynx and nose, so the larynx is called the gateway to the lungs, and the nose is the external orifice of the lungs. Fibroblasts are the main cellular components of loose connective tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and tissue defects Effect. The newly isolated lung fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. The main functions of lung fibroblasts under physiological conditions include: constructs and maintains the normal form of lung organs, synthesizes and releases extracellular matrix, and collects and repairs damaged tissues in a timely manner after tissue damage. The rat lung fibroblasts produced by our company are prepared by trypsin-collagenase mixed digestion method combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by vimentin immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.