特徴
- 背景：ProteinS（PROS1）isglycoproteinandexpressedinmanycelltypessupportingitsreportedinvolvementinmultiplebiologicalprocessesthatincludecoagulation,apoptosis,cancerdevelopmentandprogression,andtheinnateimmuneresponse.KnownreceptorsbindS1areACE2,angiotensin-convertingenzyme2,DPP4,CEACAMetc..Thespike（S）glycoproteinofcoronavirusesisknowntobeessentialinthebindingofthevirustothehostcellattheadventoftheinfectionprocess.Mostnotableissevereacuterespiratorysyndrome（SARS）.Thesevereacuterespiratorysyndrome-coronavirus（SARS-CoV）spike（S）glycoproteinalonecanmediatethemembranefusionrequiredforvirusentryandcellfusion.Itisalsoamajorimmunogenandatargetforentryinhibitors.It'sbeenreportedthat2019-nCoVcaninfectthehumanrespiratoryepithelialcellsthroughinteractionwiththehumanACE2receptor.ThespikeproteinisalargetypeItransmembraneproteincontainingtwosubunits,S1andS2.S1mainlycontainsareceptorbindingdomain（RBD）,whichisresponsibleforrecognizingthecellsurfacereceptor.S2containsbasicelementsneededforthemembranefusion.TheSproteinplayskeypartsintheinductionofneutralizing-antibodyandT-cellresponses,aswellasprotectiveimmunity.Coronavirusenvelope（E）proteinsareshort（100residues）polypeptidesthatcontainatleastonetransmembrane（TM）domainandaclusterof2-3juxtamembranecysteines.Theseproteinsareinvolvedinviralmorphogenesisandtropism,andtheirabsenceleadsinsomecasestoaberrantvirions,ortoviralattenuation.Incommontootherviroporins,coronavirusenvelopeproteinsincreasemembranepermeabilitytoions,playsacentralroleinvirusmorphogenesisandassembly.Actsasaviroporinandself-assemblesinhostmembranesformingpentamericprotein-lipidporesthatallowiontransport.Alsoplaysaroleintheinductionofapoptosis.ActivatesthehostNLRP3inflammasome,leadingtoIL-1betaoverproduction.Componentoftheviralenvelopethatplaysacentralroleinvirusmorphogenesisandassemblyviaitsinteractionswithotherviralproteins.
仕様
- サイズ：50ug
- 種由来：SARS-CoV-2
- 発現宿主：E.coli
- 純度：＞90 ％ as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションNo.：P0DTC2＆P0DTC4＆P0DTC5
- アミノ酸配列：Arg319-Phe541＆Arg38-Val75＆Arg101-Gln222
- 遺伝子構造：Recombinant SARS-CoV-2 S＆M＆E fusion is produced by E.coli expression system and the target gene encoding Arg319-Phe541＆Arg38-Val75＆Arg101-Gln222 is expressed with C-His Tag
- 別名（遺伝子名）：Spike glycoprotein;Envelope small membrane protein, E protein;Membrane protein
- 組成：Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5
- 保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
- メーカーマニュアルリンク：メーカーマニュアルはこちら
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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84-9376-16
Recombinant SARS-CoV-2 S+E+M protein 50ug
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84-9376-17
Recombinant SARS-CoV-2 S+E+M protein 100ug
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