背景：5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family.
CD73 is a glycosyl phosphatidylinositol （GPI） anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes.
CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides.
CD73 serves as a costimulatory molecule in activating T cells.
CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses.
CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function.
In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase.
CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses.
CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
仕様
サイズ：50ug
種由来：Rat
発現宿主：HEK293 Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：Q66HL0
アミノ酸配列：Met1-Lys549
遺伝子構造：A DNA sequence encoding the rat NT5E（Q66HL0） （Met1-Lys549） was expressed with a polyhistidine tag at the C-terminus.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
