背景：Mouse Sonic Hedgehog Homolog （SHH） belongs to a three-protein family called Hedgehog.
The other two family members are Indian Hedgehog （IHH） and Desert Hedgehog （DHH）.
Hedgehog proteins are key signaling molecules in embryonic development.
SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain.
SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region.
The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment （Shh-N）, and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment.
The 20 kDa Shh-N fragment is the core of the active hedgehog molecule.
Mouse Shh-N is 99％, 98％, and 100％ aa identical to human, rat and gerbil Shh-N, respectively.
仕様
サイズ：10ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞95 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：Q62226
アミノ酸配列：Cys25--Gly198
遺伝子構造：Recombinant Mouse Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys25-Gly198 is expressed with a 6His tag at the C-terminus.
別名（遺伝子名）：Sonic Hedgehog Protein; SHH; HHG-1; SHH
分子量：20.4 kDa
組成：Lyophilized from a 0.2 μm filtered solution of PBS, 5％ Trehalose, pH 7.4.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
